Beyond the DNA: The role of chromatin and lncRNAs in the regulation of alternative splicing.

Reini F. Luco Portrait de Reini Fernandez de Luco
Institute of Human Genetics, UPR 1142, CNRS, Montpellier, France
Friday 19th May 2017 at 2 pm, IBC Campus St Priest BAT5-01.124

Classically, epigenetics and chromatin conformation have been involved in the regulation of purely DNA-related processes, such as DNA transcription, replication or repair. However, it has become more and more evident that regulation of gene expression is a much more complex and multifactorial process in which chromatin is intimately implicated at multiple levels, from the DNA to the RNA. One of such processes is alternative splicing, which has been involved in cell definition and cell faith. Alternative splicing is one of the most general and important biological processes in the eukaryotic cell. It affects more than 90% of human genes, it is essential for protein diversity and any misregulation of the highly cell-specific alternative splicing programs can lead to disease, such as cancer. However the mechanisms of cell-specific alternative splicing regulation are still largely unknown.

I will first present the existing evidence for a role of chromatin, and in particular histone modifications, in the regulation of cell-specific alternative splicing.

I will then extent those evidences by showing the combinatorial role of these histone marks in splicing regulation and the importance of antisense long non-coding RNAs in the establishment of the chromatin signatures necessary for inclusion of cell-specific splicing isoforms, adding a new layer in the regulation of alternative splicing.

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